Opekarova, Miroslava and Robl, Ingrid and Grassl, Renate and Tanner, Widmar (1999) Expression of eukaryotic plasma membrane transporter HUP1 from Chlorella kessleri in Escherichia coli. FEMS MICROBIOLOGY LETTERS, 174 (1). pp. 65-72. ISSN 0378-1097, 1574-6968
Full text not available from this repository. (Request a copy)Abstract
To study the effect of sterols on the activity of the eukaryotic plasma membrane transporter, the hexose-proton symporter HUP1 from the unicellular alga Chlorella kessleri was expressed in Escherichia coli, a prokaryotic microorganism containing virtually no sterols. Under certain conditions, the recombinant protein was partially active in this prokaryotic organism. The heterologously produced HUP1p was purified from membrane fractions of E. coli and reconstituted in an in vitro system. The presence of ergosterol during solubilization, purification and reconstitution resulted in an increased activity of the reconstituted protein. Its activity, however, was 5-6 times lower as compared to the activity of HUP1p produced in Saccharomyces cerevisiae membranes and solubilized, purified, and reconstituted under the same conditions as above. (C) 1999 Published by Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; RNA-POLYMERASE; STEROLS; GENES; heterologous expression; proton-glucose symporter; reconstitution; ergosterol; Chlorella kessleri; Escherichia coli |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Oct 2022 08:50 |
| Last Modified: | 25 Oct 2022 08:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48274 |
Actions (login required)
 |
View Item |
