Ortiz, Jennifer and Stemmann, Olaf and Rank, Simone and Lechner, Johannes (1999) A putative protein complex consisting of Ctf19, Mcm21 and Okp1 represents a missing link in the budding yeast kinetochore. GENES & DEVELOPMENT, 13 (9). pp. 1140-1155. ISSN 0890-9369,
Full text not available from this repository. (Request a copy)Abstract
We have established a one-hybrid screen that allows the in vivo localization of proteins at a functional Saccharomyces cerevisiae centromere. Applying this screen we have identified three proteins-Ctf19, Mcm21 and the product of an unspecified open reading frame that we named Okp1-as components of the budding yeast centromere. Ctf19, Mcm21, and Okp1 most likely form a protein complex that links CBF3, a protein complex directly associated with the CDE III element of the centromere DNA, with further components of the budding yeast centromere, Cbf1, Mif2, and Cse4. We demonstrate that the CDE III element is essential and sufficient to localize the established protein network to the centromere and propose that the interaction of the CDE II element with the CDE III localized protein complex facilitates a protein-DNA conformation that evokes the active centromere.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE KINETOCHORE; MITOTIC CHROMOSOME TRANSMISSION; CENTROMERE PROTEIN; CROSS-LINKING; S-CEREVISIAE; HOST STRAIN; IN-VITRO; 2 GENES; CENP-C; DNA; centromere; kinetochore; CBF3; Ctf19; Mcm21; one hybrid |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Oct 2022 08:59 |
| Last Modified: | 25 Oct 2022 08:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48276 |
Actions (login required)
 |
View Item |
