Deml, Ludwig and Schirmbeck, Reinhold and Reimann, Jörg and Wolf, Hans and Wagner, Ralf (1999) Purification and characterization of hepatitis B virus surface antigen particles produced in Drosophila Schneider-2 cells. JOURNAL OF VIROLOGICAL METHODS, 79 (2). pp. 205-217. ISSN 0166-0934,
Full text not available from this repository. (Request a copy)Abstract
The small surface antigen of hepatitis B virus (HBV) was produced in Drosophila melanogaster Schneider-2 (DS-2) cells transfected stably using an inducible Drosophila metallothionein promoter. Selected clonal DS-2 cell-lines expressed and secreted large quantities of HBsAg particles consisting exclusively of non-glycosylated 25 kDa proteins. HBsAg produced by DS-2 cells had physical and biochemical properties very similar to 22 nm particles derived from the human hepatoma cell-line PLC/PRF/5. DS-2 cell-derived HBsAg particles were purified near homogeneity by a strategy based on protein concentration, precipitation and ultracentrifugation. The resulting HBsAg product was < 98% pure. A single immunisation of BALB/c mice with both DS-2 and yeast-cell derived purified HBsAg particles without adjuvants elicited a substantial humoral antibody and class-I restricted cytotoxic T lymphocyte (CTL) response. Adsorbtion of HBsAg particles to aluminium hydroxide resulted in increased levels of HBsAg-specific antibodies. However, CTLs were not elicited by HBsAg/Alum combinations. Thus, stably transfected DS-2 cells provide a useful source for the production of HBV subviral particles for diagnostic and research purposes as well as for novel vaccine development. (C) 1999 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTOTOXIC T-LYMPHOCYTES; HANSENULA-POLYMORPHA; INSECT CELLS; EXPRESSION; MICE; VACCINE; YEAST; IMMUNOGENICITY; IMMUNIZATION; RESPONSES; Drosophila Schneider-2 cells; HBsAg; expression antigenicity; immunogenicity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Oct 2022 10:31 |
| Last Modified: | 25 Oct 2022 10:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48293 |
Actions (login required)
 |
View Item |
