Doerflinger, Ulrike and Pscherer, Armin and Moser, Markus and Ruemmele, Petra and Schuele, Roland and Buettner, Reinhard (1999) Activation of somatostatin receptor II expression by transcription factors MIBP1 and SEF-2 in the murine brain. MOLECULAR AND CELLULAR BIOLOGY, 19 (5). pp. 3736-3747. ISSN 0270-7306, 1098-5549
Full text not available from this repository. (Request a copy)Abstract
Somatostatin receptor type II expression in the mammalian brain displays a spatially and temporally very restricted pattern. In an investigation of the molecular mechanisms controlling these patterns, we have recently shown that binding of the transcription factor SEF-2 to a novel initiator element in the SSTR-2 promoter is essential for SSTR-2 gene expression. Further characterization of the promoter identified a species-conserved TC-rich enhancer element. By screening a mouse brain cDNA expression library, we cloned a cDNA encoding the transcription factor MIBP1. MIBP1 interacts specifically with both the TC box in the SSTR-2 promoter and with the SEF-2 initiator-binding protein to enhance transcription from the basal SSTR-2 promoter. We then investigated SSTR-2, SEF-2, and MIBP1 mRNA expression patterns in the developing and adult murine brain by Northern blotting and in situ hybridization. While SEF-2 is widely expressed in many neuronal and nonneuronal tissues, MIBP1 expression overlapped precisely with expression of SSTR-2 in the frontal cortex and hippocampus. In summary, our data for the first time define a regulatory role for the transcription factor MIBP1 in mediating spatially and temporally regulated SSTR-2 expression in the brain.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RAT-BRAIN; MOLECULAR-CLONING; BINDING-PROTEIN; MESSENGER-RNAS; ADULT-RAT; GENE; ENHANCER; FAMILY; SUBTYPES; IMMUNOREACTIVITY; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Oct 2022 12:35 |
| Last Modified: | 25 Oct 2022 12:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48296 |
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