Comparative synthesis and hydrolytic degradation of poly (L-malate) by myxomycetes and fungi

Rathberger, Klaus and Reisner, Hermine and Willibald, Bertram and Molitoris, Hans-Peter and Holler, Eggehard (1999) Comparative synthesis and hydrolytic degradation of poly (L-malate) by myxomycetes and fungi. MYCOLOGICAL RESEARCH, 103. pp. 513-520. ISSN 0953-7562,

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Abstract

Various fungi and myxomycetes have been tested for their synthesis and hydrolytic degradation of beta-poly(L-malate) (PMLA), a highly anionic polyester of L-malate. The PMLA content of the culture medium, and also of some cell extracts, has been assayed by L-malate dehydrogenase (EC 1.1.1.83)-catalysed reduction of NAD(+) after the alkaline hydrolysis of the polymer. All eight myxomycete isolates were producers. Eight producers were Mitosporic fungi but only one Ascomyete was productive. Aureobasidium pullulans contained PMLA in yeast-like cells but not in hyphae. The polymer was bound to membrane fractions from where it was slowly released during preparation for analysis. Only 12 of the 237 fungi and myxomycetes showed PMLA-hydrolase activity; and two of these were producers of PMLA. Fungi with hydrolase activity were mostly different from those releasing L-malate. The Physarum polycephalum-type unbranched polymer of high molecular mass was not recognized in fungi. Fungi, typically A. pullulans, produced polymer of low molecular mass which seemed to be covalently bound to polysaccharide.

Item Type: Article
Uncontrolled Keywords: DNA-POLYMERASE-ALPHA; PHYSARUM-POLYCEPHALUM; AUREOBASIDIUM-PULLULANS; BETA-POLY(L-MALATE); PLASMODIA; POLYMALATASE; HYDROLASE;
Subjects: 500 Science > 570 Life sciences
500 Science > 580 Botanical sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Hans Peter Molitoris
Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eggehard Holler
Depositing User: Dr. Gernot Deinzer
Date Deposited: 25 Oct 2022 13:19
Last Modified: 25 Oct 2022 13:19
URI: https://pred.uni-regensburg.de/id/eprint/48300

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