Schraivogel, Daniel and Schindler, Susann G. and Danner, Johannes and Kremmer, Elisabeth and Pfaff, Janina and Hannus, Stefan and Depping, Reinhard and Meister, Gunter (2015) Importin-beta facilitates nuclear import of human GW proteins and balances cytoplasmic gene silencing protein levels. NUCLEIC ACIDS RESEARCH, 43 (15). pp. 7447-7461. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
MicroRNAs (miRNAs) guide Argonaute (Ago) proteins to distinct target mRNAs leading to translational repression and mRNA decay. Ago proteins interact with a member of the GW protein family, referred to as TNRC6A-C in mammals, which coordinate downstream gene-silencing processes. The cytoplasmic functions of TNRC6 and Ago proteins are reasonably well established. Both protein families are found in the nucleus as well. Their detailed nuclear functions, however, remain elusive. Furthermore, it is not clear which import routes Ago and TNRC6 proteins take into the nucleus. Using different nuclear transport assays, we find that Ago as well as TNRC6 proteins shuttle between the cytoplasm and the nucleus. While import receptors might function redundantly to transport Ago2, we demonstrate that TNRC6 proteins are imported by the Importin-beta pathway. Finally, we show that nuclear localization of both Ago2 and TNRC6 proteins can depend on each other suggesting actively balanced cytoplasmic Ago - TNRC6 levels.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN ARGONAUTE PROTEINS; MESSENGER-RNA DECAY; MEDIATED TRANSLATIONAL REPRESSION; CRYSTAL-STRUCTURE; LOCALIZATION SIGNAL; STRUCTURAL BASIS; MAMMALIAN-CELLS; PAZ DOMAIN; SLICER ACTIVITY; NONCODING RNA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2019 12:00 |
| Last Modified: | 07 Jun 2019 12:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4840 |
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