Hupf, Harald and Grimm, Daniela and Riegger, Guenter A. J. and Schunkert, Heribert (1999) Evidence for a vasopressin system in the rat heart. CIRCULATION RESEARCH, 84 (3). pp. 365-370. ISSN 0009-7330,
Full text not available from this repository. (Request a copy)Abstract
Traditionally, a hypothalamo-neurohypophysial system is thought to be the exclusive source of arginine vasopressin (AVP), a potent antidiuretic, vasoconstricting, and growth-stimulating neuropeptide. We have identified de novo synthesis of AVP in the heart as well as release of the hormone into the cardiac effluents. Specifically, molecular cloning of sequence tags amplified from isolated, buffer-perfused, and pressure-overloaded rat hearts allowed the detection of cardiac AVP mRNA, Subsequent experiments revealed a prominent induction of AVP mRNA (peak at 120 minutes, 59-fold, P<0.01 versus baseline) and peptide (peak at 120 minutes, Ii-fold, P<0.01 versus baseline) in these isolated hearts. Newly induced vasopressin peptide was localized most prominently to endothelial cells and vascular smooth muscle cells of arterioles and perivascular tissue using immunohistochemistry. In addition to pressure overload, nitric oxide (NO) participated in these alterations, because inhibition of NO synthase by N-omega-nitro-L-arginine methyl ester markedly depressed cardiac AVP mRNA and peptide induction. Immediate cardiac effects related to cardiac AVP induction in isolated, perfused, pressure-overloaded hearts appeared to be coronary vasoconstriction and impaired relaxation. These functional changes were observed in parallel with AVP induction and largely prevented by addition of a V-1 receptor blocker (10(-8) mol/L [deamino-Pen(1), O-Me-Tyr(2), Arg(8)]-vasopressin) to the perfusion buffer. Even more interesting, pressure-overloaded, isolated hearts released the peptide into the coronary effluents, offering the potential for systemic actions of AVP from cardiac origin. We conclude that the heart, stressed by acute pressure overload or NO, expresses vasopressin in concentrations sufficient to cause local and potentially systemic effects.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ARGININE-VASOPRESSIN; ENDOTHELIAL-CELLS; GENE-EXPRESSION; ANGIOTENSIN-II; SMOOTH-MUSCLE; LOCALIZATION; RADIOIMMUNOASSAY; RESISTANCE; INDUCTION; TOLERANCE; gene expression; pressure overload; heart; nitric oxide; vasoconstriction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2022 15:34 |
| Last Modified: | 15 Nov 2022 15:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48514 |
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