Mayr, Bernhard and Poetter, E. and Goretzki, P. and Rueschoff, J. and Dietmaier, W. and Hoang-Vu, C. and Dralle, H. and Brabant, G. (1999) Expression of wild type ret, ret/PTC and ret/PTC variants in papillary thyroid carcinoma in Germany. LANGENBECKS ARCHIVES OF SURGERY, 384 (1). pp. 54-59. ISSN 1435-2443,
Full text not available from this repository. (Request a copy)Abstract
Introduction: Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy. Although the clinical course is usually rather benign, a subset of tumors is more aggressive. The ret/PTC oncogene was found only in PTC, with varying frequencies of up to 30% Recently, two new variants of ret/PTC could be identified in post-Chernobyl PTCs, which raised the possibility that the prevalence of ret/PTC in non-radiation-induced PTCs might be higher than previously described. Normal thyroid cells do not express wild-type ret, but there is evidence that ret activation from any cause, including wildtype ret, occurs in more than a half of papillary tumors. Methods: We used reverse transcription polymerase chain reaction and sequencing to examine wild-type ret and all five forms of ret/PTC known today in 99 PTCs from Hannover, Dusseldorf, Halle and Regensburg. Our method could also detect other variants within the known breakpoint regions. The presence of the ret tyrosine-kinase domain was examined by immunohistochemistry. Results. Seven PTC1-positive tumors and one PTC3-positive tumor (8%), but none with the new variants or other variants of PTC1, 2 or 3 could be detected. Of 43 tumors examined, 20 showed expression of wild-type ret mRNA and staining of ret protein located predominantly to the cell membrane. Conclusion: Variants of ret/PTC do not substantially contribute to non-radiation-related ret/PTC-positive tumors, and the prevalence of ret/PTC in Germany is low in contrast to the high rate of wild-type ret expression. Thus, expression of wild-type rct should be examined for pathogenic, prognostic and possible therapeutic implications.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TYROSINE KINASE; MOLECULAR CHARACTERIZATION; ONCOGENE REARRANGEMENTS; TRANSGENIC MICE; E-CADHERIN; ACTIVATION; RECEPTOR; PROTOONCOGENE; PROTEIN; FORM; ret protooncogene; ret/PTC oncogene; thyroid papillary carcinoma |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2022 13:23 |
| Last Modified: | 22 Nov 2022 13:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48560 |
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