Zahn, Martin and Renken, Joachim and Seeger, Stefan (1999) Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers. FEBS LETTERS, 443 (3). pp. 337-340. ISSN 0014-5793,
Full text not available from this repository. (Request a copy)Abstract
A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time, Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells, Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density. (C) 1999 Federation of European Biochemical Societies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LASER; PARTICLES; BIOSENSOR; TRAP; optical tweezers; cellular screening; single cell technique; cell sensor; intracellular calcium |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2022 14:51 |
| Last Modified: | 22 Nov 2022 14:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48577 |
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