Ansell, Richard J. and Small, David A. P. and Lowe, Christopher R. (1999) The interactions of artificial coenzymes with alcohol dehydrogenase and other NAD(P)(H) dependent enzymes. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 6 (1-2). pp. 111-123. ISSN 1381-1177,
Full text not available from this repository.Abstract
The interactions of CLA, a biomimetic analogue of NAD(+) comprising a nicotinamide functionality coupled via a triazine ring to a dibenzenesulphonic acid unit, and of a series of analogues, with HLADH and other dehydrogenases have been compared to those of the natural coenzymes NAD(P)(+). CIA, together with one analogue with one of the sulphonic acid groups shifted by one position and another analogue with a single benzenedisulphonic acid unit, have been shown to be functional mimics of NAD(+) in the oxidation of butan-1-ol by horse Liver alcohol dehydrogenase (HLADH). A combination of discontinuous HPLC-based assays and continuous fluorescence based assays were used to deduce approximate kinetic constants for this reaction, using the artificial coenzymes, at pH 7.5 and 37 degrees C. HLADH demonstrated a V-max with the most active analogue which was 4% of that with NAD(+). The substrate specificity of HLADH using these coenzymes was found to change relative to that using the natural coenzyme. Activity was sought from a range of other dehydrogenases: Bacillus megaterium glucose dehydrogenase, Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase and sheep liver sorbitol dehydrogenase; all displayed activity using a range of the biomimetic coenzymes. (C) 1999 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; REDOX COENZYME; DYE TEMPLATE; NICOTINAMIDE; ANALOGS; GLUCOSE; CONFORMATION; NAD(P)(H); biomimetic coenzyme; coenzyme analogue; dehydrogenases; coenzyme binding; horse liver alcohol dehydrogenase |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 22 Nov 2022 15:36 |
| Last Modified: | 22 Nov 2022 15:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48589 |
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