Kullmann, Frank and Judex, Martin and Ballhorn, Wibke and Juesten, Hans-Peter and Wessinghage, Dieter and Welsh, John and Yen, Tim J. and Lang, Bernhard and Hittle, Jim C. and McClelland, Michael and Gay, Steffen and Schoelmerich, Juergen and Mueller-Ladner, Ulf (1999) Kinesin-like protein CENP-E is upregulated in rheumatoid synovial fibroblasts. ARTHRITIS RESEARCH, 1 (1). pp. 71-80. ISSN 1465-9913,
Full text not available from this repository.Abstract
Our aim was to identify specifically expressed genes using RNA arbitrarily primed (RAP)polymerase chain reaction (PCR) for differential display in patients with rheumatoid arthritis (RA). In RA, amplification of a distinct PCR product suitable for sequencing could be observed. Sequence analysis identified the PCR product as highly homologous to a 434 base pair segment of the human centromere kinesin-like protein CENP-E. Differential expression of CENP-E was confirmed by quantitative reverse transcription PCR, immunohistochemistry and in situ hybridization. CENP-E expression was independent from prednisolone and could not be completely inhibited by serum starvation. RAP-PCR is a suitable method to identify differentially expressed genes in rheumatoid synovial fibroblasts. Also, because motifs of CENP-E show homologies to jun and fos oncogene products and are involved in virus assembly, CENP-E may be involved in the pathophysiology of RA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; arthritis; centromere; differential display; immunohistochemistry; in situ hybridization; RNA fingerprinting |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Orthopädie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2022 09:49 |
| Last Modified: | 29 Nov 2022 09:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48612 |
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