Koncki, Robert and Wolfbeis, Otto S. (1999) Composite films of Prussian blue and N-substituted polypyrroles: covalent immobilization of enzymes and application to near infrared optical biosensing. BIOSENSORS & BIOELECTRONICS, 14 (1). pp. 87-92. ISSN 0956-5663,
Full text not available from this repository. (Request a copy)Abstract
We demonstrate the feasibility of optical biosensing using a material which, in essence, is a modified inorganic film to which various enzymes were covalently attached. Thin and transparent blue films composed of Prussian blue and incorporated into a network of N-substituted polypyrroles are sensitive to pH in the 5-9 range at 720 nm wavelength and can be modified with enzymes to result in the respective biosensors. Several methods of enzyme immobilization, using bifunctional crosslinking reagents, and various enzymes were tested. The best results were obtained using the one-step carbodiimide method which resulted in highly active, stable and transparent biosensor films for optical determination of urea and acetylcholine. The operational stability exceeded 1 month and even after 2 months of dry storage at room temperature the activity did not drop. The biosensors allow optical determination of the respective substrates in the millimolar concentration range. (C) 1999 Elsevier Science S.A. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FLOW-INJECTION ANALYSIS; FIELD-EFFECT TRANSISTOR; AMPEROMETRIC BIOSENSORS; SENSORS; PENICILLIN; GLUCOSE; ELECTRODES; enzyme immobilization; films; optical biofilms; polypyrroles; Prussian blue |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2022 10:08 |
| Last Modified: | 29 Nov 2022 10:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48619 |
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