Schmalz, Gottfried and Schuster, Uta and Nuetzel, Katja and Schweikl, Helmut (1999) An in vitro pulp chamber with three-dimensional cell cultures. JOURNAL OF ENDODONTICS, 25 (1). pp. 24-29. ISSN 0099-2399,
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To better simulate the in vivo situation, a three-dimensional fibroblast cell culture was introduced into an in vitro pulp chamber model. The system was evaluated by testing a series of dental filling materials. After a 24-h exposure with (0.3 or 5 ml/h) and without perfusion of the pulp chamber, the tissues were subjected to a routine MTT assay. Zinc phosphate cement, conventional glass ionomer cements, a silicone impression material, and zinc oxide-eugenol did not influence cell viability, compared with untreated controls; but, a light-curing glass ionomer cement significantly reduced cell survival. Perfusion of the chambers did not significantly influence the results, but perfusion conditions of 5 ml/h lead to a general decrease of cell vitality. The three-dimensional cell culture system in an in vitro pulp chamber seems to be a substantial improvement, because zinc oxide-eugenol does not evoke a cellular reaction (as is the case in vivo), and the test system is sensitive enough to detect other toxicants.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYTO-TOXICITY; HUMAN-DENTIN; CYTOTOXICITY; INVITRO; ALLOYS; MODEL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2022 16:17 |
| Last Modified: | 29 Nov 2022 16:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48667 |
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