Arndt, Holger and Kullmann, Frank and Reuss, Friedrich and Schoelmerich, Juergen and Palitzsch, Klaus-Dieter (1999) Glutamine attenuates leukocyte-endothelial cell adhesion in indomethacin-induced intestinal inflammation in the rat. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 23 (1). pp. 12-18. ISSN 0148-6071,
Full text not available from this repository.Abstract
Background: Glutamine (Gln) is a major energy source for the intestinal mucosa. Its depiction results in epithelial atrophy and in bacterial translocation. Clinical substitution of this nonessential amino acid in critically ill persons results in a reduction of epithelial atrophy and in an accelerated recovery. The objective of this study was to assess the effect of Gin on leukocyte-endothelial cell interaction in an indomethacin (Indo)-induced long-lasting ileitis in Sprague-Dawley rats. Methods: Indo (7.5 mg/kg subcutaneously) was injected at time 0 and 24 hours later. Animals were fed with standard rat chow (ST) for 10 days until 12 hours before intravital microscopy analysis. Gin (3 g/kg body wt) was gavaged twice a day in the morning 4 hours apart(1) for 10 days between Indo administration and the experiment (ST/Gln, therapy), (2) for 14 days before Indo (Gln/ST, prophylaxis), or (3) from 14 days before Indo until the experiment (Gln/Gln, prophylaxis and therapy). Ten mesenteric venules (30 mu m diameter) per animal(n = 5 per group) were observed using intravital microscopy, and the following parameters were monitored: number of adherent and emi grated leukocytes, leukocyte rolling velocity, erythrocyte velocity, venular blood flow, and shear rate. Macroscopically visible injury was scored 0 to 5. Results: Ten days after Indo treatment the macroscopic score was 3.5 +/- 0.4 vs 0.6 +/-. 0.2 of controls, and leukocyte adherence and emigration were increased (2.2-fold and 3.3-fold vs control, respectively), whereas leukocyte rolling velocity and venular wall shear rate were reduced (both parameters to 81% of control). Glutamine prophylaxis, therapy, and the combination of both significantly attenuated macroscopic damage and prevented the microcirculatory disturbances to a similar extent. The beneficial effects of glutamine were accompanied by a normalization of fecal pH to control level, which had been lowered by Indo treatment. Conclusions: The long-lasting Indo-induced ileitis was accompanied by macroscopic ulceration and microcirculatory disturbances. Oral therapy and prophylaxis with glutamine reduced macroscopic and microcirculatory inflammatory activity, indicating a special demand for glutamine in this type of inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TOTAL PARENTERAL-NUTRITION; IMMUNE-SYSTEM; AMINO-ACIDS; MUCOSA; TOXICITY; THERAPY; INJURY; MUSCLE; SEPSIS; ENZYME; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Nov 2022 09:35 |
| Last Modified: | 30 Nov 2022 09:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48679 |
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