Farkas, Stefan and Herfarth, H. and Guba, M. and Steinbauer, M. and Scholmerich, J. and Jauch, Karl-Walter and Anthuber, M. (1999) ICAM-1 antibodies reduce leukocyte adherence and -extravasation in DSS-induced colitis in mice: assessment by in vivo fluorescence microscopy. LANGENBECKS ARCHIVES OF SURGERY. pp. 403-406. ISSN 1435-2443,
Full text not available from this repository. (Request a copy)Abstract
Introduction: Leuko cyte-endothelial interactions play a pivotal role in the pathogenesis of inflammatory bowel disease (IBD). In addition recent research suggest an important role for the adhesionmolecule ICAM-1 in intestinal inflammation. Therefore we investigated the effect of an monoclonar antibody against ICAM-1 on leukocyte adherence and extravasation in colitis using in vivo fluorescence microscopy. Material and methods: Colitis was established in Balb C mice by DSS (Dextran Sodium Sulfate) application. Controls received isotyp control antibody, the experimental group anti ICAM-1 antibody. In vivo microscopy was performed after mobilizing the colon and in vivo labelling of leukocytes venule of the colon was calculated. After antimesenteric incision the number of extravasated leukocytes in the mucosa was counted ICAM-1 expression was demonstrated by immunhistochemistry. Results: ICAM-1 expression was increased in DSS-induced colitis. Treatment with anti ICA M-l antibody reduced leukocyte adherence in submucosal postcapillary and collecting venules and leukocytes extravasation in the mucosa compared to controls. Summary and Conclusion: We demonstrated increased ICAM-1 expression in DSS-induced colitis in mice. Using in vivo microscopy, we further were able to show that a monoclonal antibody against ICAM-1 significantly reduces leukocyte adherance and extravasation in DSS-induced colonic inflammation. The results support not only the therapeutical concept of ICAM-1 suppression in IBD [2] but also establish the method of in vivo microscopy as a new tool for the evaluation of experimental therapies in colonic : inflammation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ADHESION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Nov 2022 09:49 |
| Last Modified: | 30 Nov 2022 09:49 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48687 |
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