Imhof, Axel and Schuierer, Marion and Werner, Oliver and Moser, Markus and Roth, Christina and Bauer, Reinhard and Buettner, Reinhard (1999) Transcriptional regulation of the AP-2 alpha promoter by BTEB-1 and AP-2rep, a novel wt-1/egr-related zinc finger repressor. MOLECULAR AND CELLULAR BIOLOGY, 19 (1). pp. 194-204. ISSN 0270-7306,
Full text not available from this repository. (Request a copy)Abstract
AP-2 transcription factors have been suggested to exert key regulatory functions in vertebrate embryonic development, in tumorigenicity of various cancer cell types, and in controlling cell cycle and apoptotic effector genes. In this study, we investigated transcriptional regulation of the AP-2 alpha gene promoter mediated by an autoregulatory element (referred to as A32) with a core consensus AP-2 binding site at position -336 relative to the mRNA initiation site. AP-2 and multiple different nuclear proteins in HeLa and Neuro2A cell extracts form specific bandshifts with the A32 element. By screening a mouse brain cDNA expression library, we isolated two different cDNAs encoding the transcription factor BTEB-1 and a novel zinc finger protein, AP-2rep. AP-2rep reveals a modular structure with homology to transcription factors of the wt-1/egr-1-family. AP-2rep, BTEB-1, and AP-2 interact in a mutually exclusive manner with overlapping binding sites in the A32 element. Transfection studies revealed that BTEB-1 is a strong activator of AP-2 alpha promoter activity, whereas cotransfected AP-2 alpha resulted in moderate autoactivation of promoter activity. In contrast, AP-2rep confers strong transcriptional repression to the AP-2 alpha gene, and we observed an excellent correlation between induction of AP-2rep mRNA expression and downregulation of AP-2 alpha mRNA during development of the kidney. In summary, we have identified multiple transcription factors and cloned from an expression library a novel zinc finger silencing factor, AP-2rep, mediating positive and negative regulation of AP-2 alpha expression through a set of overlapping cis-regulatory promoter elements.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-BINDING; MOUSE EMBRYOGENESIS; GENE-EXPRESSION; ACTIVATION; AP-2-BETA; REGION; CELLS; CARCINOMA; SEQUENCE; PROTEINS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Dec 2022 07:26 |
| Last Modified: | 05 Dec 2022 07:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48706 |
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