Baumann, Rosemarie and Blass, Christian and Goetz, Robert and Dragon, Stefanie (1999) Ontogeny of catecholamine and adenosine receptor-mediated cAMP signaling of embryonic red blood cells: Role of cGMP-inhibited phosphodiesterase 3 and hemoglobin. BLOOD, 94 (12). pp. 4314-4320. ISSN 0006-4971,
Full text not available from this repository. (Request a copy)Abstract
We have previously shown that the cAMP signaling pathway controls major aspects of embryonic red blood cell (RBC) function in avian embryos (Glombitza et al, Am J Physiol 271:R973, 1996; and Dragon et al, Am J Physiol 271:R982, 1996) that are important for adaptation of the RBC gas transport properties to the progressive hypercapnia and hypoxia of later stages of avian embryonic development. Data about the ontogeny of receptor-mediated cAMP signaling are lacking. We have analyzed the response of primitive and definitive chick embryo RBC harvested from day 3 to 18 of development towards forskolin, beta-adrenergic, and A2 receptor agonists. The results show a strong response of immature definitive and primitive RBC to adenosine A2 and beta-adrenergic receptor agonists, which is drastically reduced in the last stage of development, coincident with the appearance of mature, transcriptionally inactive RBC. Modulation of cGMP-inhibited phosphodiesterase 3 (PDE3) has a controlling influence on cAMP accumulation in definitive RBC. Under physiological conditions, PDE3 is inhibited due to activation of soluble guanylyl cyclase (sGC), Inhibition of sGC with the specific inhibitor ODQ decreases receptor-mediated stimulation of cAMP production; this effect is reversed by the PDE3 inhibitor milrinone. sGC is acitivated by nitric oxide (NO), but we found no evidence for production of NO by erythrocyte NO-synthase. However, embryonic hemoglobin releases NO in an oxygen-linked manner that may activate guanylyl cyclase. (C) 1999 by The American Society of Hematology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AVIAN EMBRYO; OXYGEN; ERYTHROCYTES; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Alumni or Retired > Prof. Dr. Rosemarie Baumann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Dec 2022 09:26 |
| Last Modified: | 06 Dec 2022 09:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48764 |
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