Takeuchi, Sousuke and Imafuku, Ichiro and Waragai, Masaaki and Roth, Christina and Kanazawa, Ichiro and Buettner, Reinhard and Mouradian, M. Maral and Okazawa, Hitoshi (1999) AP-2 beta represses D-1A dopamine receptor gene transcription in Neuro2a cells. MOLECULAR BRAIN RESEARCH, 74 (1-2). pp. 208-216. ISSN 0169-328X,
Full text not available from this repository. (Request a copy)Abstract
Expression of the D-1A dopamine receptor in brain is restricted to specific neuronal populations. To investigate the mechanism of this selective expression, we localized a silencer upstream of the human D-1A gene and identified its binding transcription factor in the D-1A-negative neural cell line Neuro2a. Using deletion CAT analysis, we narrowed this silencer to the region between nucleotides -561 and -532 relative to the CAP site. This 30-bp region, designated D1AS1, contains a sequence homologous to the AP-2 binding site and binds to a factor that also interacts with the AP-2 consensus sequence. In gel supershift assays, this factor is recognized by anti-AP-2 beta antibody. Co-transfection of Neuro2a cells with an AP-2 beta expression vector repressed the basal CAT activity of D-1A promoter-reporter plasmids in a D1AS1-dependent manner. RT-PCR analysis indicated that, among AP-2 family members, Neuro2a cells express only AP-2 beta. Furthermore, co-transfection of these cells with decoy oligonucleotides corresponding to the D1AS1 sequence de-repressed the D-1A gene promoter. Unlike in Neuro2a cells, AP-2 beta could not repress the D-1A promoter in the D-1A-positive neural cell line, NS20Y. in addition, the expression of AP-2 beta in different brain regions does not inversely correlate with that of D-1A dopamine receptor. These observations taken together indicate that AP-2 beta is a repressive transcription factor that acts on the D1AS1 silencer of the D-1A dopamine receptor gene via some cell-specific mechanism(s) in Neuro2a. (C) 1999 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RETINOIC ACID; ENHANCER; IDENTIFICATION; EXPRESSION; CLONING; AP-2; DIFFERENTIATION; AP-2-ALPHA; PROTEIN; FAMILY; AP-2 beta; D-1A dopamine receptor; gene regulation; silencer; repressor; differentiation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Dec 2022 09:52 |
| Last Modified: | 06 Dec 2022 09:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48770 |
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