Lu, Lichun and Kam, Lance and Hasenbein, Meredith and Nyalakonda, Kavita and Bizios, Rena and Goepferich, Achim and Young, James F. and Mikos, Antonios G. (1999) Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces. BIOMATERIALS, 20 (23-24). pp. 2351-2361. ISSN 0142-9612, 1878-5905
Full text not available from this repository. (Request a copy)Abstract
Model substrates with desired chemical micropatterns were fabricated using a microcontact printing technique. The substrate surfaces contained organized arrays of circular glass domains with a diameter of either 10 or 50 mu m surrounded and separated by regions modified with octadecyltrichlorosilane (OTS) self-assembled monolayers (SAMs). The effects of surface patterning on in vitro cell attachment, proliferation, morphology, and cytoskeletal organization were evaluated using a human retinal pigment epithelium (RPE) cell line. Both micropatterns affected initial RPE cell attachment, limited cell spreading, and promoted the characteristic cuboidal cell morphology throughout the culture period. In contrast, RPE cells on plain glass control were elongated and appeared fibroblast-like prior to confluence. In addition, cells seeded at 30 000 cell/cm(2) on the patterned surfaces maintained a normal pattern of actin and cytokeratin expression, and formed confluent monolayers within 4 days of culture. The cell density increased about 30-fold on both micropatterns by day 7. These results show that it is feasible to control RPE cell shape and expression of differentiated phenotype using micropatterned surfaces. (C) 1999 Elsevier Science Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MACULAR DEGENERATION; RCS RATS; TRANSPLANTATION; CULTURE; POLARITY; MONKEY; SHAPE; STAMP; micropatterned surface; retinal pigment epithelium; cell function; microcontact printing; self-assembled monolayer |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Dec 2022 10:40 |
| Last Modified: | 06 Dec 2022 10:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48774 |
Actions (login required)
 |
View Item |
