New approach to fluorescence spectroscopy of individual molecules on surfaces

Enderlein, Joerg (1999) New approach to fluorescence spectroscopy of individual molecules on surfaces. PHYSICAL REVIEW LETTERS, 83 (19). pp. 3804-3807. ISSN 0031-9007, 1079-7114

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Abstract

A new method for efficient fluorescence excitation of molecules on a surface is proposed and theoretically studied. A laser beam is focused into a diffraction limited spot on a thin layer of an optically nonlinear material. The frequency doubled light that is generated within that layer is used to excite the fluorescence of molecules positioned above the layer. It is shown that such an excitation scheme may be more efficient than two-photon excitation. This may be of great importance in single molecule fluorescence detection at short wavelengths.

Item Type: Article
Uncontrolled Keywords: SINGLE MOLECULES; 2-PHOTON EXCITATION; MICROSCOPY; LIQUIDS; PHOTON; SOLIDS; FILMS;
Subjects: 500 Science > 540 Chemistry & allied sciences
Divisions: Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik
Depositing User: Dr. Gernot Deinzer
Date Deposited: 13 Dec 2022 06:38
Last Modified: 13 Dec 2022 06:38
URI: https://pred.uni-regensburg.de/id/eprint/48844

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