Pallares, Fernando Perez de los Cobos and Stanic, Davor and Farmer, David and Dutschmann, Mathias and Egger, Veronica (2015) An arterially perfused nose-olfactory bulb preparation of the rat. JOURNAL OF NEUROPHYSIOLOGY, 114 (3). pp. 2033-2042. ISSN 0022-3077, 1522-1598
Full text not available from this repository. (Request a copy)Abstract
A main feature of the mammalian olfactory bulb network is the presence of various rhythmic activities, in particular, gamma, beta, and theta oscillations, with the latter coupled to the respiratory rhythm. Interactions between those oscillations as well as the spatial distribution of network activation are likely to determine olfactory coding. Here, we describe a novel semi-intact perfused nose-olfactory bulb-brain stem preparation in rats with both a preserved olfactory epithelium and brain stem, which could be particularly suitable for the study of oscillatory activity and spatial odor mapping within the olfactory bulb, in particular, in hitherto inaccessible locations. In the perfused olfactory bulb, we observed robust spontaneous oscillations, mostly in the theta range. Odor application resulted in an increase in oscillatory power in higher frequency ranges, stimulus-locked local field potentials, and excitation or inhibition of individual bulbar neurons, similar to odor responses reported from in vivo recordings. Thus our method constitutes the first viable in situ preparation of a mammalian system that uses airborne odor stimuli and preserves these characteristic features of odor processing. This preparation will allow the use of highly invasive experimental procedures and the application of techniques such as patch-clamp recording, high-resolution imaging, and optogenetics within the entire olfactory bulb.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LONG-LASTING DEPOLARIZATIONS; ACTION-POTENTIAL PROPAGATION; BRAIN-STEM PREPARATION; MITRAL CELLS; GRANULE CELLS; IN-VITRO; GABAERGIC INTERNEURONS; RESPIRATORY NEURONS; SENSORY INFORMATION; GAMMA-OSCILLATIONS; neural oscillation; theta rhythm; olfactory bulb; odor coding; brain stem |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Neurophysiologie (Prof. Dr. Veronica Egger) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 11 Jun 2019 13:45 |
| Last Modified: | 11 Jun 2019 13:45 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4887 |
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