Obermeier, Florian and Gross, Volker and Schoelmerich, Juergen and Falk, Werner (1999) Interleukin-1 production by mouse macrophages is regulated in a feedback fashion by nitric oxide. JOURNAL OF LEUKOCYTE BIOLOGY, 66 (5). pp. 829-836. ISSN 0741-5400,
Full text not available from this repository.Abstract
The pleiotropic cytokine interleukin-1 (IL-1) is an inducer of the inducible nitric oxide synthase (iNOS). It was surprising to find that treatment of normal mice with an iNOS inhibitor resulted in detectable IL-1 beta mRNA in colon and spleen, suggesting feedback regulation. When mouse peritoneal exudate cells (PEC) or RAW 264.7 cells were stimulated with lipopolysaccharide (LPS), concomitant inhibition of iNOS resulted in an increase of IL-1 beta and IL-1 alpha protein secretion. Conversely, after addition of the NO-generating compound NOC-18, IL-1 beta and IL-1 alpha concentrations in supernatants were dose-dependently reduced. Costimulation with interferon-gamma (IFN-gamma) reversed the NOC-18-mediated suppression of IL-1 alpha. protein concentration into an almost fivefold increase in RAW 264.7 cells. This effect was specific for IL-1 alpha and was also seen in PEG. The mRNA expression for IL-1 beta and IL-1 alpha. in RAW 264.7 cells correlated with the protein. levels, suggesting transcriptional regulation by NO. Dysregulated IL-1/NO cross-regulation may play a role in inflammatory diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NF-KAPPA-B; ULCERATIVE-COLITIS; SYNTHASE EXPRESSION; MURINE MACROPHAGES; INTERFERON-GAMMA; IMMUNE-RESPONSES; L-ARGININE; CELL-LINE; MICE; INHIBITION; interferon-gamma; interleukin-1 alpha; interleukin-1 beta; peritoneal exudate cells; RAW 264.7; lipopolysaccharide |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Dec 2022 09:14 |
| Last Modified: | 13 Dec 2022 09:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48883 |
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