Synthesis and characterization of new chloride-sensitive indicator dyes based on dynamic fluorescence quenching

Huber, Christian and Faehnrich, Karsten and Krause, Christian and Werner, Tobias (1999) Synthesis and characterization of new chloride-sensitive indicator dyes based on dynamic fluorescence quenching. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 128 (1-3). pp. 111-120. ISSN 1010-6030,

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Abstract

New water-soluble chloride-sensitive indicator dyes based on a bisacridine backbone were synthesized. These quaternized bisacridinium derivatives and the commercial available lucigenin are compared with acridinium compounds in terms of absorbance, fluorescence excitation and emission spectra, their fluorescence decay time and Stern-Volmer constants (K-SV) for quenching by a series of anions. All these dyes are quenched dynamically on exposure to chloride and obey the Stem-Volmer law. The effect of pH, buffer composition and ionic strength on the quenching mechanism was investigated. (C) 1999 Elsevier Science S.A. All rights reserved.

Item Type: Article
Uncontrolled Keywords: PROBE; fluorescence quenching; chloride probe; bisacridinium derivatives
Subjects: 500 Science > 540 Chemistry & allied sciences
Divisions: Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 13 Dec 2022 09:24
Last Modified: 13 Dec 2022 09:24
URI: https://pred.uni-regensburg.de/id/eprint/48887

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