Hofmann, Markus and Ehness, Rainer and Lee, Task Kyun and Roitsch, Thomas (1999) Intracellular protons are not involved in elicitor dependent regulation of mRNAs for defence related enzymes in Chenopodium rubrum. JOURNAL OF PLANT PHYSIOLOGY, 155 (4-5). pp. 527-532. ISSN 0176-1617,
Full text not available from this repository.Abstract
Protons have been implicated in the signal transduction of elicitor-generated defence reactions. To address the possible function of cytosolic acidification we determined both intracellular and extracellular pH values and phenylalanine-ammonia-lyase (PAL) and extracellular invertase (CIN1) mRNA levels of photoautotrophic suspension culture Chenopodium rubrum cells in response to a number of stimuli known to elicit defence responses. The results of these experiments show that there is no principle link between elicitor-induced persistent pH-shifts and the induction of the defence related mRNAs. Treatment with the fungal elicitor chitosan led to intracellular acidification, extracellular alkalinisation, and a rise of both PAL and CIN1 mRNA levels. However, when these pH shifts are reversed by treatment with fusicoccin, a known activator of the plasma membrane ATPase, both PAL and CIN 1 mRNA levels are also elevated. A comparison between the kinetics of intracellular acidification and the rise in PAL and CIN 1 mRNA levels in response to chitosan shows that the activation of these genes precedes intracellular acidification by 1 h, indicating that protons are not involved in the analysed signal transduction pathway leading to the induction of PAL and CIN 1 mRNAs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXTRACELLULAR ALKALINIZATION; TOBACCO CELLS; CYTOPLASMIC ACIDIFICATION; PROTEIN-PHOSPHORYLATION; SUSPENSION-CULTURES; SOYBEAN CELLS; TOMATO CELLS; PLANT-CELLS; PH; ELICITATION; Chenopodium rubrum; elicitor; intracellular pH; extracellular pH; signal transduction |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Dec 2022 15:28 |
| Last Modified: | 20 Dec 2022 15:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48962 |
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