Ruppert, Claudia and Kavermann, Holger and Wimmers, Sönke and Schmid, Roland and Kellermann, Joseph and Lottspeich, Friedrich and Huber, Harald and Stetter, Karl Otto and Mueller, Volker (1999) The proteolipid of the A(1)A(0) ATP synthase from Methanococcus jannaschii has six predicted transmembrane helices but only two proton-translocating carboxyl groups. JOURNAL OF BIOLOGICAL CHEMISTRY, 274 (36). pp. 25281-25284. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
The proteolipid, a hydrophobic ATPase subunit essential for ion translocation, was purified from membranes of Methanococcus jannaschii by chloroform/methanol extraction and gel chromatography and was studied using molecular and biochemical techniques. Its apparent molecular mass as determined in SDS-polyacrylamide gel electrophoresis varied considerably with the conditions applied. The N-terminal sequence analysis made it possible to define the open reading frame and revealed that the gene is a triplication of the gene present in bacteria. In some of the proteolipids, the N-terminal methionine is excised. Consequently, two forms with molecular masses of 21,316 and 21,183 Da were determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The molecular and biochemical data gave clear evidence that the mature proteolipid from M. jannaschii is a triplication of the 8-kDa proteolipid present in bacterial F1F0 ATPases and most archaeal A(1)A(0) ATPases, Moreover, the triplicated form lacks a proton-translocating carboxyl group in the first of three pairs of transmembrane helices, This finding puts in question the current view of the evolution of H+ ATPases and has important mechanistic consequences for the structure and function of H+ ATPases in general.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | METHANOSARCINA-MAZEI GO1; COENZYME-M METHYLTRANSFERASE; ESCHERICHIA-COLI; STRAIN GO1; F-0 SECTOR; ARCHAEON; STOICHIOMETRY; ORGANIZATION; EXPRESSION; EVOLUTION; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2022 06:58 |
| Last Modified: | 21 Dec 2022 06:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49002 |
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