Mackensen, Andreas and Wittnebel, S. and Veelken, H. and Noppen, C. and Spagnoli, G. C. and Lindemann, A. (1999) Induction and large-scale expansion of CD8(+) tumor specific cytotoxic T lymphocytes from peripheral blood lymphocytes by in vitro stimulation with CD80-transfected autologous melanoma cells. EUROPEAN CYTOKINE NETWORK, 10 (3). pp. 329-335. ISSN 1148-5493,
Full text not available from this repository.Abstract
Human melanoma cell lines may induce a specific T cell response against tumor cells in vitro. However, after repeated restimulation with autologous tumor cells, expansion of CTL is limited and often apoptosis of the T cells occurs. In order to improve conditions inducing primary T cell responses and thus allowing further expansion of tumor specific T cells for an adoptive transfer, we transfected human melanoma cells with the B7.1 gene (CD80), known to be a potent costimulatory molecule for T cell activation. CD80 expression on melanoma cells resulted in improved primary T cell activation, especially of CD8(+) T cells. Furthermore, restimulation with CD80(+) tumor cells gave rise to long term proliferating CD8(+) T cell lines demonstrating an 100-fold expansion of T cells compared to the 20-30-fold increased numbers obtained with the controls (parental tumor cells +/- anti-CD28). T cells stimulated with CD80(+) melanoma cells were found to display a MHC class I-restricted cytotoxic activity against autologous tumor cells. In conclusion, these studies demonstrate the requirement of costimulation in generating large numbers of tumor specific T cells in vitro that may be used for an adoptive transfer in tumor immunotherapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ALLOGENEIC BONE-MARROW; IN-VITRO; COSTIMULATION; IMMUNITY; B7-1; GENERATION; INTERLEUKIN-12; IMMUNIZATION; LIGAND; DONOR; melanoma; B7; CTL; adoptive immunotherapy |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2022 09:38 |
| Last Modified: | 21 Dec 2022 09:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49011 |
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