von Landenberg, Christine and Lackner, Karl J. and von Landenberg, Philipp and Lang, Bernhard and Schmitz, Gerd (1999) Isolation and characterization of two human monoclonal anti-phospholipid IgG from patients with autoimmune disease. JOURNAL OF AUTOIMMUNITY, 13 (2). pp. 215-223. ISSN 0896-8411,
Full text not available from this repository. (Request a copy)Abstract
The antigenic specificity of anti-phospholipid antibodies (APA) is a matter of intensive investigation. To further characterize these antibodies,we attempted to isolate human monoclonal APA. B-cells of patients with at least one positive test for antibodies against cardiolipin, phosphatidylserine, beta 2-glycoprotein I (beta 2-GPI) or the lupus anti-coagulant were immortalized by transformation with Epstein-Barr virus and screened for production of specific IgG. Positive pools were fused with a heteromyeloma cell line and APA-secreting clones were isolated by standard procedures. Two monoclonal APA, HL-SB from a 51-year-old man with primary anti-phospholipid syndrome and recurrent cerebral microinfarctions, and RR-7F from a 48-year-old women with systemic lupus erythematosus but no evidence for thrombotic events were obtained. HL-5B is of the IgG2 subtype with lambda light chains, while RR-7F is IgG2 with kappa light chains. Both monoclonals show reactivity against cardiolipin and phosphatidylserine but lack reactivity against beta 2-GPI or lupus anti-coagulant activity. To yield the same OD in the cardiolipin and phosphatidylserine ELISAs RR-7F must be used in an approximately 10-fold higher concentration than HL-5B, indicating a lower affinity towards these antigens. Interestingly, both mAPA can bind to cardiolipin in the absence of beta 2-GPI. They do not cross-react with dsDNA but show reactivity against oxidized low-density lipoproteins. Analysis of the heavy chain mRNA of HL-5B and RR-7F showed that both are members of the VH3 family. While HL-5B shows extensive somatic mutations in the CDR1 and 2 regions, indicating that it was derived by a T cell-dependent antigen driven process, RR-7F is apparently germline encoded. The two monoclonal APA can be used as tools in further structural and functional analyses. (C) 1999 Academic Press.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SYSTEMIC LUPUS-ERYTHEMATOSUS; EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME; ANTICARDIOLIPIN ANTIBODIES; ANTI-BETA(2)-GLYCOPROTEIN-I ANTIBODIES; ENDOTHELIAL-CELLS; BINDING; BETA(2)-GLYCOPROTEIN-I; AUTOANTIBODIES; CARDIOLIPIN; THROMBOSIS; anti-phospholipid antibody; human; monoclonal |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2022 16:04 |
| Last Modified: | 21 Dec 2022 16:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49029 |
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