Stoitzner, Patrizia and Zanella, Monica and Ortner, Ulrike and Lukas, Michael and Tagwerker, Andrea and Janke, Katrin and Lutz, Manfred B. and Schuler, Gerold and Echtenacher, Bernd and Ryffel, Bernhard and Koch, Franz and Romani, Nikolaus (1999) Migration of Langerhans cells and dermal dendritic cells in skin organ cultures: augmentation by TNF-alpha and IL-1 beta. JOURNAL OF LEUKOCYTE BIOLOGY, 66 (3). pp. 462-470. ISSN 0741-5400,
Full text not available from this repository. (Request a copy)Abstract
Migration from sites of antigen encounter to lymphoid organs is essential to the strong immunogenic function of dendritic cells (DC), In the skin, migration proceeds through dermal lymphatic vessels and is regulated in an incompletely understood way by inflammatory mediators, We studied the effects of tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) in mouse skin organ cultures by direct enumeration of migrating DC and by immunohistochemistry. (1) Neutralizing antibodies to TNF-alpha and IL-1 beta inhibited migration of DC, also in human skin explants (TNF-alpha). (2) TNF-alpha at low concentrations (50 U/mL) anti TL-1 beta (50-3000 U/mL) augmented migration to about 150% of spontaneous migration (3) High concentrations of TNF-alpha (5000 U/mL) inhibited migration by approximately 50%. (4) DC migration from skin explants of TNF-alpha/lymphotoxin-alpha double-deficient mice and TNF-receptor type 1 and 2 double knock out mice was not impaired. (5) TNF-alpha effects were neutralized by anti-IL-1 beta, and vice versa, We conclude that in normal animals both TNF-alpha and IL-1 beta are required for BC migration to occur. In the complete absence of one cytokine (TNF-alpha), however, backup mechanisms step in.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; COLONY-STIMULATING FACTOR; ANTIGEN-PRESENTING CELLS; CONTACT HYPERSENSITIVITY; FUNCTIONAL-CHARACTERIZATION; MONOCLONAL-ANTIBODY; DEFICIENT MICE; I RECEPTOR; E-CADHERIN; MATURATION; lymphatic vessels; inflammatory mediators; immunohistochemistry |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Dec 2022 16:26 |
| Last Modified: | 21 Dec 2022 16:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49039 |
Actions (login required)
 |
View Item |
