RFLP analysis as a tool for identification of lignicolous basidiomycetes: European polypores

Fischer, M. and Wagner, T. (1999) RFLP analysis as a tool for identification of lignicolous basidiomycetes: European polypores. EUROPEAN JOURNAL OF FOREST PATHOLOGY, 29 (4). pp. 295-304. ISSN 0300-1237,

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Abstract

Restriction fragment length polymorphisms were generated for 52 species of lignicolous European polypores belonging to 31 different genera. An enzymatically amplified portion of the nuclear-encoded ribosomal DNA was found to be about 1800-1900 bp long for most taxa. After digestion with Hpa II, 44 distinct phenotypes were detected for the species under study; application of the additional restriction endonucleases, Hin6 I and Hinf I, resulted in 48 species-specific and two genera-specific phenotypes. The procedure described should allow for a reliable identification of unknown mycelia of lignicolous fungi.

Item Type: Article
Uncontrolled Keywords: AMPLIFIED RIBOSOMAL DNA; PHELLINUS; PCR; SEQUENCES; SPACERS;
Subjects: 500 Science > 580 Botanical sciences
Divisions: Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften
Depositing User: Dr. Gernot Deinzer
Date Deposited: 11 Jan 2023 10:19
Last Modified: 11 Jan 2023 10:19
URI: https://pred.uni-regensburg.de/id/eprint/49084

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