In vivo observation of cell division of anaerobic hyperthermophiles by using a high-intensity dark-field microscope

Horn, Christian and Paulmann, Bernd and Kerlen, Gertraude and Junker, Norbert and Huber, Harald (1999) In vivo observation of cell division of anaerobic hyperthermophiles by using a high-intensity dark-field microscope. JOURNAL OF BACTERIOLOGY, 181 (16). pp. 5114-5118. ISSN 0021-9193,

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Abstract

To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98 degrees C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a modified dark-field microscope, the formation of cannulae, which finally connected all cells, was observed. The cannulae elongated at 1.0 to 1.5 mu m/min and reached final lengths of between 30 and 150 mu m. A "snapping division"-like mode of cell fission was discovered for Thermoproteus tenux.

Item Type: Article
Uncontrolled Keywords: 105-DEGREES-C; NOV;
Subjects: 500 Science > 570 Life sciences
Divisions: Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 11 Jan 2023 10:53
Last Modified: 11 Jan 2023 10:53
URI: https://pred.uni-regensburg.de/id/eprint/49090

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