Homberg, Uwe and Vitzthum, Harm and Mueller, Monika and Binkle, Ulrike (1999) Immunocytochemistry of GABA in the central complex of the locust Schistocerca gregaria: Identification of immunoreactive neurons and colocalization with neuropeptides. JOURNAL OF COMPARATIVE NEUROLOGY, 409 (3). pp. 495-507. ISSN 0021-9967,
Full text not available from this repository. (Request a copy)Abstract
The central complex is a highly organized neuropil structure in the insect brain and plays a role in motor control and visual orientation. We describe the distribution of gamma-aminobutyric acid (GABA) immunostaining in the central complex of the locust Schistocerca gregaria in an effort to analyze inhibitory neural circuits within this brain area. Antisera against GABA and the GABA-synthesizing enzyme glutamic acid decarboxylase resulted in identical patterns of immunostaining. Cell counts revealed about 100 bilateral pairs of GABA-immunoreactive neurons with arborizations in the central complex. Five types of immunostained neurons could be identified through reconstruction of the staining pattern, comparison with individually stained neurons, and double labeling experiments with Neurobiotin-injected neurons. All of these GABA-immunostained neurons are tangential neurons that connect the lateral accessory lobes to distinct layers of the central body. Three types of immunostained neurons (TL2, TL3, TL4) invade the lower division of the central body, and two additional types of neurons (TU1, TU2) have ramifications in layers I and II of the upper division of the central body. Double-labeling experiments with peptide antisera suggest that peptides related to Phe-Met-Arg-Phe-NH(2)/bovine pancreatic polypeptide and Dip-allatostatin might act as cotransmitters with GABA in TL4 neurons of the lower division and (Dip-allatostatin only) in TU2 neurons of the upper division of the central body. The high conservation in the pattern of GABA immunostaining in all insect species investigated so far suggests that GABA plays an essential role in the basic neural circuitry of the central complex in insects. (C) 1999 Wiley-Liss, Inc.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLUTAMIC-ACID DECARBOXYLASE; COLORADO POTATO BEETLE; CENTRAL-NERVOUS-SYSTEM; DROSOPHILA-MELANOGASTER; PERIPLANETA-AMERICANA; FMRFAMIDE ANTISERUM; MANDUCA-SEXTA; DESERT LOCUST; BRAIN; PEPTIDES; insect brain; central body; gamma-aminobutyric acid; allatostatin; FMRF amide |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2023 10:22 |
| Last Modified: | 31 Jan 2023 10:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49134 |
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