Ludwig, Claudia and Ecker, Susanne and Schwindel, Kerstin and Rast, Hans-Georg and Stetter, Karl Otto and Eberz, Günther (1999) Construction of a highly bioluminescent Nitrosomonas as a probe for nitrification conditions. ARCHIVES OF MICROBIOLOGY, 172 (1). pp. 45-50. ISSN 0302-8933,
Full text not available from this repository. (Request a copy)Abstract
Cloned luciferase-encoding operons were transferred by conjugation to a natural isolate of the ammonia-oxidizing bacterial strain Nitrosomonas sp. RST41-3, thereby establishing conjugation as a tool for gene transfer into Nitrosomonas strains. Luminescence was dependent on the pH of the medium and the concentration of the substrate ammonium chloride. Moreover, the luminescence of the transconjugants was reduced immediately by micromolar concentrations of nitrapyrin and allylthiourea, which are specific inhibitors of nitrification. Our results indicate that luminescent Nitrosomonas strains may be useful as a probe to detect nitrification conditions in the natural environment as well as in sewage plants.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AMMONIA-OXIDIZING BACTERIA; GRAM-NEGATIVE BACTERIA; BROAD HOST RANGE; ALCALIGENES-EUTROPHUS; CLONING VECTORS; PLASMID; GENES; MOBILIZATION; MUTAGENESIS; INHIBITORS; ammonia oxidation; bioluminescence; conjugation; nitrification inhibitor; Nitrosomonas |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2023 11:08 |
| Last Modified: | 31 Jan 2023 11:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49141 |
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