Piletsky, Sergey A. and Terpetschnig, Ewald and Andersson, Hakan S. and Nicholls, Ian A. and Wolfbeis, Otto S. (1999) Application of non-specific fluorescent dyes for monitoring enantio-selective ligand binding to molecularly imprinted polymers. FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 364 (6). pp. 512-516. ISSN 0937-0633,
Full text not available from this repository. (Request a copy)Abstract
The displacement of non-specific dyes from molecularly imprinted polymer (MIP) chromatographic stationary phases has been used for the detection and quantification of ligand-polymer binding events. A blank polymer and an L-phenylalaninamide-imprinted polymer were prepared using methacrylic acid as the functional monomer and ethylene glycol dimethacrylate as a crosslinker. The MIP is first loaded with dye, and a solution of the dye in the eluent is passed through the MIP. If analyte is injected into the dye solution in the eluent, part of the dye is competitively replaced by the analyte from the MIP. Specifically, the competitive displacement of rhodamine B by amino acids and phenylalaninamide (Phe-NH2), respectively, has been studied under polar and hydrophobic elution conditions. Enantioselective binding of Phe and Phe-NH2 to the imprinted polymer was shown to occur in the micromolar concentration range. It is proposed that the displacement of non-specific dyes from MIPs be used for the development of multisensors based upon these highly specific and stable materials, which provide promising alternatives to the use of biological macromolecules in sensor technology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | OPTICAL-DETECTION; CHROMATOGRAPHY; RECOGNITION; BIOSENSORS; MEMBRANES; PROTEINS; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2023 11:20 |
| Last Modified: | 31 Jan 2023 11:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49147 |
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