Stoyan, Tanja and Eck, Raimund and Lechner, Johannes and Hemmerich, Peter and Kuenkel, Waldemar and Diekmann, Stephan (1999) Cloning of a centromere binding factor 3d (CBF3D) gene from Candida glabrata. YEAST, 15 (9). pp. 793-798. ISSN 0749-503X,
Full text not available from this repository. (Request a copy)Abstract
The gene encoding centromere binding factor 3d (CBF3D) of the human pathogenic yeast Candida glabrata has been isolated by hybridization of Saccharomyces cerevisiae CBF3D (ScCBF3D) DNA to a C. glabrata partial genomic library. Sequence analysis revealed a 540 bp open reading frame encoding a protein of 179 amino acids with a calculated molecular mass of 20.9 kDa. The amino acid sequence is highly homologous (78.6% identity) to ScCbf3d and 48.3% identical to the human homologue p19 (SKP1). Southern blot analysis indicates that CgCbf3d is encoded by an unique nuclear gene. The cloned CgCBF3D gene can functionally substitute the S. cerevisiae homologue in a S. cerevisiae CBF3D-deletion mutant. The GenBank Accession No. for this gene is AF 072472. Copyright (C) 1999 John Wiley & Sons, Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE KINETOCHORE; KLUYVEROMYCES-LACTIS; BUDDING YEAST; CONSTRUCTION; HOMOLOGY; DNA; RESISTANCE; SELECTION; SEQUENCE; ELEMENTS; Candida glabrata; CBF3D; centromere |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2023 14:38 |
| Last Modified: | 31 Jan 2023 14:38 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49161 |
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