Schuler, Benjamin and Rachel, Reinhard and Seckler, Robert (1999) Formation of fibrous aggregates from a non-native intermediate: The isolated P22 tailspike beta-helix domain. JOURNAL OF BIOLOGICAL CHEMISTRY, 274 (26). pp. 18589-18596. ISSN 0021-9258,
Full text not available from this repository. (Request a copy)Abstract
In the assembly pathway of the trimeric P22 tailspike protein, the protein conformation critical for the partitioning between productive folding and off-pathway aggregation is a monomeric folding intermediate. The central domain of tailspike, a large right-handed parallel beta-helix, is essentially structured in this species, We used the isolated beta-helix domain (Bhx), expressed with a hexahistidine tag, to investigate the mechanism of aggregation without the two terminal domains present in the complete protein. Although Bhx has been shown to fold reversibly at low ionic strength conditions, increased ionic strength induced aggregation with a maximum at urea concentrations corresponding to the midpoint of urea-induced folding transitions. According to size exclusion chromatography, aggregation appeared to proceed via a linear polymerization mechanism. Circular dichroism indicated a secondary structure content of the aggregates similar to that of the native state, but at the same time their tryptophan fluorescence was largely quenched. Microscopic analysis of the aggregates revealed a variety of morphologies; among others, fibrils with fine structure were observed that exhibited bright green birefringence if viewed under cross-polarized light after staining with Congo red, These observations, together with the effects of folding mutations on the aggregation process, indicate the involvement of a partially structured intermediate distinct from both unfolded and native Bhx.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SENSITIVE FOLDING MUTATIONS; INCLUSION-BODY FORMATION; HEAD-BINDING DOMAIN; TAIL-SPIKE PROTEIN; ALTERNATIVE CONFORMATIONS; AMYLOIDOGENIC PROTEINS; ELECTRON-MICROSCOPY; POLYPEPTIDE-CHAINS; CRYSTAL-STRUCTURE; MOLECULAR-BASIS; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Jan 2023 14:47 |
| Last Modified: | 31 Jan 2023 14:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49162 |
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