Sanders, Sylvia L. and Gentzsch, Martina and Tanner, Widmar and Herskowitz, Ira (1999) O-glycosylation of Axl2/Bud10p by Pmt4p is required for its stability, localization, and function in daughter cells. JOURNAL OF CELL BIOLOGY, 145 (6). pp. 1177-1188. ISSN 0021-9525, 1540-8140
Full text not available from this repository. (Request a copy)Abstract
Cells of the yeast Saccharomyces cerevisiae choose bud sites in a manner that is dependent upon cell type: a and alpha cells select axial sites: a/alpha cells utilize bipolar sites. Mutants specifically defective in axial budding were isolated from an a strain using pseudohyphal growth as an assay. We found that a and alpha mutants defective in the previously identified PMT4 gene exhibit unipolar, rather than axial budding: mother cells choose axial bud sites, but daughter cells do not. PMT4 encodes a protein mannosyl transferase (pmt) required for O-linked glycosylation of some secretory and cell surface proteins (Immervoll,T.. M. Gentzsch, and W. Tanner. 1995. Yeast. 11:1345-1351).We demonstrate that Ax12/Bud10p, which is required for the axial budding pattern, is an O-linked glycoprotein and is incompletely glycosylated, unstable, and mislocalized in cells lacking PMT4. Overexpression of AXL2 can partially restore proper bud-site selection to pmt4 mutants. These data indicate that Axl2/Bud10p is glycosylated by Pmt4p and that O-linked glycosylation increases Ax12/Bud10p activity in daughter cells, apparently by enhancing its stability and promoting its localization to the plasma membrane.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BUD-SITE SELECTION; YEAST SACCHAROMYCES-CEREVISIAE; SERINE-RICH REGION; LINKED GLYCOSYLATION; SECRETORY PATHWAY; ANCHORED PROTEIN; GENETIC-CONTROL; KEX2 PROTEASE; GROWTH; SURFACE; O-glycosylation; PMT4; AXL2/BUD10; budding; polarity |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Feb 2023 10:14 |
| Last Modified: | 01 Feb 2023 10:14 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49172 |
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