Christgau, Michael and Caffesse, Raul G. and Newland, J. Robert and Schmalz, Gottfried and D'Souza, Rena N. (1998) Characterization of immunocompetent cells in the diseased canine periodontium. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 46 (12). pp. 1443-1454. ISSN 0022-1554,
Full text not available from this repository. (Request a copy)Abstract
The beagle dog with naturally occurring periodontal disease is one of the most widely used animal models in periodontal research for histological studies on disease pathogenesis and on the effect of potential therapeutic regimens. However, previous studies were restricted to morphological assessment of immunocompetent cells because of the lack of available cell-specific markers. In this study we systematically characterized the specificity and immunoreactivity of a panel of anti-human antibodies for identification (ABC method) of immunocompetent cells in formalin-fixed, EDTA-decalcified, paraffin-embedded inflamed periodontal tissues obtained from six beagle dogs. Canine lymph nodes and a panel of different human tissues served as positive controls. Polyclonal anti-CD3 immunolabeled canine T-lymphocytes specifically. Anti-CD79 alpha (clone HM57) reacted with B-lymphocytes and plasma cells, and CD79 alpha (clone JCP117) showed no staining in canine tissues. Neutrophils, monocytes, small macrophages, and keratinocytes reacted with an anti-myeloid/ histiocyte antibody (clone MAC387). Anti-CD68 (clones PG-M1 and EBM11) immunolabeled large macrophages and plasma cells. Clone EBM11 also stained osteoclasts and cementoclasts. With the exception of JCB117, all antibodies revealed similarly favorable immunolabeling of canine and human immunocompetent cells. Long-term EDTA decalcification appeared to weaken immunostaining of plasma cells with HM57. MAC387 and CD68 can be used to distinguish macrophages in different differentiation stages in canine periodontal tissues.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MONOCLONAL-ANTIBODY EBM/11; HOST RESPONSE; INFLAMMATORY CELLS; PARAFFIN SECTIONS; BIOPSY SPECIMENS; CD3 ANTIGEN; T-CELLS; B-CELLS; EXPRESSION; MB-1; immunohistochemistry; dogs; periodontal disease; macrophages; monocytes; T-lymphocytes; B-lymphocytes; plasma cells; neutrophils; osteoclasts |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Feb 2023 07:35 |
| Last Modified: | 21 Feb 2023 07:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49302 |
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