Lueck, P. Christian and Schmitt, Jürgen W. and Hengerer, Arne and Helbig, Jürgen H. (1998) Subinhibitory concentrations of antimicrobial agents reduce the uptake of Legionella pneumophila into Acanthamoeba castellanii and U937 cells by altering the expression of virulence-associated antigens. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 42 (11). pp. 2870-2876. ISSN 0066-4804,
Full text not available from this repository.Abstract
We determined the MICs of ampicillin, ciprofloxacin, erythromycin, imipenem, and rifampin for two clinical isolates of Legionella pneumophila serogroup 1 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay and by quantitative culture, To test the influence of subinhibitory concentrations (sub-MICs) of antimicrobial agents on Legioneila uptake into Acanthamoeba castellanii and U937 macrophage-like cells, both strains were pretreated with 0.25 MICs of the antibiotics for 24 h, In comparison to that for the untreated control, subinhibitory concentrations of antibiotics significantly reduced Legionella uptake into the host cells, Measurement of the binding of monoclonal antibodies against several Legionella antigens by enzyme-linked immunoassays indicated that sub-MIG antibiotic treatment reduced the expression of the macrophage infectivity potentiator protein (Mip), the Hsp 60 protein, the outer membrane protein (OmpM), an as-yet-uncharacterized protein of 55 kDa, and a few lipopolysaccharide (LPS) epitopes, In contrast, the expression of some LPS epitopes recognized by monoclonal antibodies 8/5 and 30/4 as well as a 45-kDa protein, a 58-kDa protein, and the major outer membrane protein (OmpS) remained unaffected.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SUB-INHIBITORY CONCENTRATIONS; OUTER-MEMBRANE PROTEIN; ESCHERICHIA-COLI; MONOCLONAL-ANTIBODIES; LEGIONNAIRES-DISEASE; NUCLEOTIDE-SEQUENCE; HUMAN MONOCYTES; MIP PROTEINS; IN-VITRO; ANTIBIOTICS; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Mar 2023 14:29 |
| Last Modified: | 28 Mar 2023 14:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49369 |
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