Wagner, Charlotte and Pfeifer, A. and Ruth, P. and Hofmann, F. and Kurtz, Armin (1998) Role of cGMP-kinase II in the control of renin secretion and renin expression. JOURNAL OF CLINICAL INVESTIGATION, 102 (8). pp. 1576-1582. ISSN 0021-9738, 1558-8238
Full text not available from this repository. (Request a copy)Abstract
To investigate the roles of the cGMP-dependent protein kinases (cGKs) in the control of the renin system, we studied the regulation of renin in cGKI- or cGKII-deficient mice in vivo and in vitro. Renal renin mRNA levels both under stimulatory (low-salt diet plus ramipril) and inhibitory (high-salt diet) conditions were not different between wildtype and cGKI-/- mice, but were significantly elevated in cGKII-/- mice under all experimental conditions. In primary cultures of renal juxtaglomerular cells (JG) established from wild-type, cGKI-/-, and cGKII-/- mice, the adenylate cyclase activator forskolin stimulated renin secretion similarly in all genotypes tested. 8-bromo-cGMP attenuated basal and forskolin-stimulated renin secretion in cultures from wild-type and cGKI-/-, but had no effect in cells isolated from cGKII-/- mice. Activation of cGKs by 8-bromo-cGMP decreased renin secretion from the isolated perfused rat kidney, independent of prestimulation by beta-adrenoreceptor activation, macula densa inhibition, reduced perfusion pressure, or by a nominally calcium-free perfusate. Taken together, these findings suggest that activation of cGKII has a general inhibitory effect on renin secretion from renal JG cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT PROTEIN-KINASE; ATRIAL-NATRIURETIC-PEPTIDE; NITRIC-OXIDE SYNTHESIS; JUXTAGLOMERULAR CELLS; RELAXING FACTOR; PRESSURE CONTROL; GENE-EXPRESSION; CONSCIOUS DOGS; PHOSPHATASE 2A; RAT-KIDNEY; renin mRNA; juxtaglomerular cell; isolated perfused kidney; cGKII; knockout |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Apr 2023 10:40 |
| Last Modified: | 18 Apr 2023 10:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49432 |
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