Schütz, M. and Klughammer, Christof and Griesbeck, Christoph and Quentmeier, Armin and Friedrich, Cornelius G. and Hauska, Günter (1998) Sulfide-quinone reductase activity in membranes of the chemotrophic bacterium Paracoccus denitrificans GB17. ARCHIVES OF MICROBIOLOGY, 170 (5). pp. 353-360. ISSN 0302-8933, 1432-072X
Full text not available from this repository.Abstract
Reduction of exogenous ubiquinone and of cytochromes by sulfide in membranes of the chemotrophic bacterium Paracoccus denitrificans GB17 was studied. For sulfide-ubiquinone reductase activity, K-m, values of 26 +/- 4 and 3.1 +/- 0.6 mu M were determined from titrations with sulfide and decyl-ubiquinone, respectively. A maximal rate of up to 0.3 mu mol decyl-ubiquinone reduced (mg protein)(-1) min(-1) was estimated. The reaction was sensitive to quinone-analogous inhibitors, but insensitive to cyanide. Reduction of cytochromes by sulfide was monitored with an LED-array spectrophotometer. Under oxic conditions, reduction rates and extents of reduction were lower than those under anoxic conditions. Reoxidation of cytochromes was oxygen-dependent and cyanide-sensitive. The multiphasic behavior of transient reduction of cytochrome b with limiting amounts of sulfide reflects that sulfide, in addition to acting as an electron donor, is a slowly binding inhibitor of cytochrome c oxidase. The initial peak of cytochrome b reduction is dependent on electron flow to an oxidant, either oxygen or ferricyanide, and is stimulated by antimycin A. This oxidant-induced reduction of cytochrome b suggests that electron transport from sulfide in P. denitrificans GB17 employs the cytochrome bc(1) complex via the quinone pool.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CYANOBACTERIUM OSCILLATORIA-LIMNETICA; THIOSPHAERA-PANTOTROPHA; ANOXYGENIC PHOTOSYNTHESIS; THIOBACILLUS-FERROOXIDANS; RHODOBACTER-CAPSULATUS; ELECTRON-TRANSFER; ELEMENTAL SULFUR; OXIDATION; PURIFICATION; CYTOCHROMES; Paracoccus denitrificans; sulfide oxidation; sulfide-quinone reductase; cytochrome bc complex; flavocytochrome c |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Alumni or Retired > Prof. Dr. Günter Hauska |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2023 06:26 |
| Last Modified: | 16 Feb 2023 06:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49446 |
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