Gomes, Claudio M. and Faria, Alice and Carita, João C. and Mendes, Joaquim and Regalla, Manuela and Chicau, Paula and Huber, Harald and Stetter, Karl O. and Teixeira, Miguel (1998) Di-cluster, seven-iron ferredoxins from hyperthermophilic Sulfolobales. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 3 (5). pp. 499-507. ISSN 0949-8257, 1432-1327
Full text not available from this repository.Abstract
Seven-iron ferredoxins from the thermoacidophilic archaea Acidianus ambivalens, A. infernus, Metalosphaera prunae and Sulfolobus metallicus were extensively characterised, allowing study of their expression under aerobic and anaerobic growth conditions as well as the putative role in thermal stability of a recently described zinc centre. The archaeon S. metallicus was found to express, under the same growth conditions, two ferredoxins in almost identical amounts, a novelty among Archaea. Most interestingly, these two ferredoxins differ at the N-terminal amino acid sequence in that one has a zinc binding motif (FdA) and the other does not (FdB); in agreement with these:findings, FdA contains a zinc ion and FdB does not. These two ferredoxins have identical thermal stabilities, indicating that the zinc atom is not determinant in the protein thermostability. Further, the presence of the additional zinc centre does not interfere with the redox properties of the iron-sulfur clusters since their reduction potentials are almost identical. From the other three archaea, independently of the growth mode in respect to oxygen, only a single zinc-containing ferredoxin was found. EPR studies on the purified proteins, both in the oxidised and dithionite reduced states, allowed the identification of one [3Fe-4S](1+/0) centre and one [4Fe-4S](2+/1+) centre in all proteins studied. The complete sequence of A. ambivalens ferredoxin is reported. Together with the data gathered in this study, the properties of the seven-iron ferredoxins from Sulfolobales so far known are re-discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLETE GENOME SEQUENCE; AMINO-ACID-SEQUENCE; ARCHAEBACTERIUM PYROCOCCUS-FURIOSUS; ELECTRON-PARAMAGNETIC-RES; DESULFUROLOBUS-AMBIVALENS; SP-NOV; THERMOACIDOPHILIC ARCHAEBACTERIUM; DESULFOVIBRIO-AFRICANUS; GLUTAMATE-DEHYDROGENASE; BIOLOGICAL-ACTIVITY; ferredoxins; thermophiles; Archaea; EPR; iron-sulfur |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Feb 2023 07:15 |
| Last Modified: | 23 Feb 2023 07:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49462 |
Actions (login required)
 |
View Item |
