Neubauer, H. and Reischl, Udo and Ropp, S. and Esposito, J. J. and Wolf, H. and Meyer, H. (1998) Specific detection of monkeypox virus by polymerase chain reaction. JOURNAL OF VIROLOGICAL METHODS, 74 (2). pp. 201-207. ISSN 0166-0934,
Full text not available from this repository.Abstract
The open reading frame coding for the A-type inclusion body protein (ATI) of monkeypox virus (MPV) was identified and sequenced for two strains. Nucleotide sequence comparison revealed 72-95.3% homology with the reported open reading frame sequences of the ATIs of other orthopoxvirus species, such as variola, vaccinia, cowpox, ectromelia, and camelpox viruses. Each MPV strain contained an 8-bp deletion, which caused a frameshift that introduced a premature stop in the open reading frame at base 2091 relative to the ATI open reading frame of cowpox virus strain Brighten. The sequences enabled a primer pair to be designed that flanked the deletion and specifically amplified a 601-bp fragment that identified and differentiated 19 MPV strains examined from five other Old World orthopoxvirus species examined. The specificity was confirmed by cleavage of the 19 MPV strain amplicons with Bg/II, which produced three subfragments of expected sized, based on the determined MPV sequences. (C) 1998 Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INCLUSION PROTEIN; ORTHOPOXVIRUS; GENE; DIFFERENTIATION; BODY; orthopoxvirus; DNA sequences; acidophilic inclusion body protein |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Apr 2023 13:21 |
| Last Modified: | 18 Apr 2023 13:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49473 |
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