Gabler, Stefan and Schuett, Holger and Groitl, Peter and Wolf, Hans and Shenk, Thomas and Dobner, Thomas (1998) E1B 55-kilodalton-associated protein: a cellular protein with RNA-binding activity implicated in nucleocytoplasmic transport of adenovirus and cellular mRNAs. JOURNAL OF VIROLOGY, 72 (10). pp. 7960-7971. ISSN 0022-538X,
Full text not available from this repository. (Request a copy)Abstract
The adenovirus type 5 (Ad5) early 1B 55-kDa protein (E1B-55kDa) is a multifunctional phosphoprotein that regulates viral DNA replication and nucleocytoplasmic RNA transport in lytically infected cells. In addition, E1B-55kDa provides functions required for complete oncogenic transformation of rodent cells in cooperation with the E1A proteins. Using the far-Western technique, we have isolated human genes encoding E1B-55kDa-associated proteins (E1B-APs). The E1B-AP5 gene encodes a novel nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family that is highly related to hnRNP-U/SAF-A. Immunoprecipitation experiments indicate that two distinct segments in the 55-kDa polypeptide which partly overlap regions responsible for p53 binding are required for complex formation with E1B-AP5 in Ad-infected cells and that this protein interaction is modulated by the adenovirus E4orf6 protein. Expression of E1B-AP5 efficiently interferes with Ad5 E1A/E1B-mediated transformation of primary rat cells. Furthermore, stable expression of E1B-AP5 in Ad-infected cells overcomes the E1B-dependent inhibition of cytoplasmic host mRNA accumulation. These data suggest that E1B-AP5 might play a role in RNA transport and that this function is modulated by E1B-55kDa in Ad-infected cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LATE GENE-EXPRESSION; INFECTED HELA-CELLS; MESSENGER-RNA; NUCLEAR-MATRIX; SH3 DOMAINS; HNRNP-U; STRUCTURAL BASIS; DNA-REPLICATION; FOCUS FORMATION; TUMOR-ANTIGEN; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Apr 2023 13:23 |
| Last Modified: | 18 Apr 2023 13:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49474 |
Actions (login required)
 |
View Item |
