Stoehr, Josef and Schindler, Gisela and Rothe, Gregor and Schmitz, Gerd (1998) Enhanced upregulation of the fc gamma receptor IIIa (CD16a) during in vitro differentiation of ApoE4/4 monocytes. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 18 (9). pp. 1424-1432. ISSN 1079-5642,
Full text not available from this repository. (Request a copy)Abstract
We recently reported a positive correlation of the pool size of lipopolysaccharide receptor (CD14)(dim) and Fc gamma receptor IIIa (CD16a)(+) monocytes in peripheral blood to the apolipoprotein E4 (apoE4) phenotype and a negative correlation to high density lipoprotein (HDL) cholesterol levels (Arterioscler Thromb Vasc Biol. 1996;16:1437-1447). In this study, the in vitro differentiation of mononuclear phagocytes derived from healthy blood donors homozygous for the E3/3 or the E4/4 phenotype was analyzed during 7 days of culture in serum-free medium supplemented with macrophage colony-stimulating factor (M-CSF). The CD16a expression, which indicates Fc receptor-dependent phagocytic activity, increased to a significantly higher level in apoE4/4 monocytes than in apoE3/3 cells. The costimulatory molecule CD40, which indicates antigen-presenting capacity, was upregulated more strongly in apoE3/3 monocytes compared with E4/4 cells, but the difference did not reach a significant level. The expression of differentiation-associated surface proteins (CD14, CD33, CD45) and adhesion molecules (CD11a, CD11b, CD11c, CD49d) was not significantly different between apoE3/3 and apoE4/4 monocytes. However, a significantly decreased intracellular apoE concentration and a reduced amount of secreted apoE were found in apoE4/4 monocytes during in vitro differentiation. No differences were found in the surface expression of the low density lipoprotein receptor-related protein (CD91) and the uptake of fluorescence labeled low density lipoprotein between apoE3/3 and apoE4/4 monocytes. These data indicate that the apoE4/4 phenotype significantly influences the M-CSF-dependent differentiation of monocytes toward a more CD16a-positive phagocytic phenotype.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLONY-STIMULATING FACTOR; GROWTH-FACTOR-BETA; FAMILIAL ALZHEIMER-DISEASE; AMYLOID PRECURSOR PROTEIN; E-DEFICIENT MICE; APOLIPOPROTEIN-E; LIPOPROTEIN RECEPTOR; CARDIOVASCULAR-DISEASE; COMPLEMENT ACTIVATION; GENE-EXPRESSION; monocytes; apolipoprotein E; CD16a; CD40 low density lipoprotein receptor-related protein |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Apr 2023 09:08 |
| Last Modified: | 25 Apr 2023 09:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49523 |
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