Thies, Frank L. and Hartung, Hans-Peter and Giegerich, Gerhard (1998) Cloning and expression of the Campylobacter jejuni lon gene detected by RNA arbitrarily primed PCR. FEMS MICROBIOLOGY LETTERS, 165 (2). pp. 329-334. ISSN 0378-1097, 1574-6968
Full text not available from this repository. (Request a copy)Abstract
Fingerprinting of RNA by arbitrarily primed PCR was used to identify a heat-inducible gene in Campylobacter jejuni. Comparing RNA fingerprints from C. jejuni cells before and after 20 min of heat shock at 48 degrees C, a differentially amplified PCR product was identified which displayed a high degree of homology to bacterial lon genes. By screening C. jejuni genomic libraries, the entire lon gene was cloned and sequenced. It encodes a protein of 791 amino acids with a calculated molecular mass of 90.2 kDa. Alignment of the Lon amino acid sequence with that of other bacterial species revealed an overall identity of up to 56.6% (Helicobacter pylori). Northern and RNA dot blot experiments confirmed heat induction of the C. jejuni lon gene, revealing a maximum 6-8-fold increase in the level of specific mRNA. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ATP-DEPENDENT PROTEASE; NUCLEOTIDE-SEQUENCE; MYXOCOCCUS-XANTHUS; BACILLUS-SUBTILIS; COMPETENCE; COML; LA; Campylobacter jejuni; lon; RNA arbitrarily primed polymerase chain reaction; heat shock; ATP-dependent protease |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Neurologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Feb 2023 10:21 |
| Last Modified: | 16 Feb 2023 10:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49591 |
Actions (login required)
 |
View Item |
