Gomes, Claudio M. and Huber, Harald and Stetter, Karl O. and Teixeira, Miguel (1998) Evidence for a novel type of iron cluster in the respiratory chain of the archaeon Sulfolobus metallicus. FEBS LETTERS, 432 (3). pp. 99-102. ISSN 1873-3468
Full text not available from this repository. (Request a copy)Abstract
A new type of metal centre was detected in the membranes of the thermoacidophilic archaeon Sulfolobus metallicus. This centre has an S=1/2 ground state in the oxidised form, yielding an axial EPR signal with g values at 2.035 (g(parallel to)) and 1.97 (g(perpendicular to)), optimally detected at 4.6-10 K; in the reduced form it is EPR silent (even spin). These magnetic properties point to a spin-coupled iron cluster, with a minimum of two iron ions. The centre has a high reduction potential of + 350 mV, at pH 6.5, The physiological role of this novel centre was probed through a general characterisation of S, metallicus respiratory chain: this archaeon contains NADH and succinate dehydrogenase activities, and cytochromes b(562), a(586) and a(600) on the oxygen reductase system. Since it is reduced in the presence of succinate, and taking into account its high reduction potential, this centre is proposed to be a functional analogue of the Rieske centres, (C) 1998 Federation of European Biochemical Societies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TERMINAL OXIDASE; QUINOL OXIDASE; DESULFUROLOBUS-AMBIVALENS; ACIDIANUS-AMBIVALENS; ARCHAEBACTERIUM SULFOLOBUS; THERMOACIDOPHILIC ARCHAEON; SUCCINATE-DEHYDROGENASE; DESULFOVIBRIO-GIGAS; ALTERNATIVE OXIDASE; REDOX PROTEINS; archaeon; electron paramagnetic resonance; iron-sulphur; Rieske; terminal oxidase; thermophile |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Feb 2023 09:06 |
| Last Modified: | 23 Feb 2023 09:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49606 |
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