Löscher, F. and Böhme, S. and Martin, J. and Seeger, Stefan (1998) Counting of single protein molecules at interfaces and application of this technique in early-stage diagnosis. ANALYTICAL CHEMISTRY, 70 (15). pp. 3202-3205. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
The fluorescence-based detection and counting of single protein molecules after specific binding to antibodies at interfaces is presented. A diode laser was used as the excitation source. The unspecific binding at the interface has been reduced to a level of only 0.1% of the maximum signal level. At present, the detection limit of this molecule counting process is in the range of 10(-17) mol/L, and the dynamic range of the signal corresponds to 7 orders of magnitude of antigen concentration, but these values are not limiting. As a preliminary application in early-stage diagnosis, we have investigated the detection of a single cardiac actin molecule in human plasma, which is of interest in myocardial infarction diagnosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CONFOCAL FLUORESCENCE MICROSCOPY; OPTICAL-DETECTION; HIGH-SENSITIVITY; SPECTROSCOPY; SOLIDS; LASER; IMMOBILIZATION; ABSORPTION |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Feb 2023 09:18 |
| Last Modified: | 23 Feb 2023 09:18 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49615 |
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