Rogler, Gerhard and Brand, Korbinian and Vogl, Daniela and Page, Sharon and Hofmeister, Robert and Andus, Tilo and Knuechel, Ruth and Baeuerle, Patrick A. and Schoelmerich, Juergen and Gross, Volker (1998) Nuclear factor kappa B is activated in macrophages and epithelial cells of inflamed intestinal mucosa. GASTROENTEROLOGY, 115 (2). pp. 357-369. ISSN 0016-5085,
Full text not available from this repository. (Request a copy)Abstract
Background & Aims: Transcription factors of the nuclear factor kappa B (NF-kappa B) family play an important role in the regulation of genes involved in inflammation. In inflammatory bowel diseases, proinflammatory cytokines known to be regulated by NF-kappa B are involved. The aim of this study was to investigate the role of NF-kappa B activation during mucosal inflammation in situ. Methods: A monoclonal antibody, alpha-p65mAb, was applied for immunofluorescence and immunohistochemical analysis that recognizes activated NF-kappa B. Electrophoretic mobility shift assay was used to directly demonstrate the presence of active DNA-binding NF-kappa B. Results: Using the alpha-p65mAb antibody, activated NF-kappa B could be found in biopsy specimens from inflamed mucosa but was almost absent in uninflamed mucosa. The number of cells showing NF-kappa B activation correlated with the degree of mucosal inflammation but was not significantly different between inflamed mucosa from patients with Crohn's disease, ulcerative colitis, and nonspecific colitis or diverticulitis. NF-kappa B activation was localized in macrophages and in epithelial cells as identified by double-labeling techniques. Electrophoretic mobility shift assay with isolated lamina propria mononuclear cells and epithelial cells confirmed these results. Conclusions: This study shows for the first time the activation of NF-kappa B during human mucosal inflammation in situ. In addition to macrophages, epithelial cells contained activated NF-kappa B, indicating an involvement in the inflammatory process.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INFLAMMATORY BOWEL-DISEASE; NECROSIS-FACTOR-ALPHA; TRANSCRIPTION FACTOR; LAMINA PROPRIA; MONOCLONAL-ANTIBODY; ULCERATIVE-COLITIS; GENE-EXPRESSION; CROHNS-DISEASE; IEC-6 CELLS; INTERLEUKIN-8; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 May 2023 09:41 |
| Last Modified: | 02 May 2023 09:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49626 |
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