Wagner, Charlotte and Kurtz, Armin (1998) Positive feedback regulation of angiotensin II AT(1B) receptor gene expression in rat adrenal glands. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 436 (3). pp. 323-328. ISSN 0031-6768, 1432-2013
Full text not available from this repository.Abstract
This study aimed to investigate the role of endogenous angiotensin II (ANGII) in the upregulation of ANG-II AT(1) receptors in adrenal glands during a low-salt intake. To this end male Sprague-Dawley rats were fed a low-salt diet (0.2 mg/g) for 10 days and were treated with the ANGII-AT(1) receptor antagonist losartan (40 mg/kg per day) for 2 days, and adrenal mRNA levels for ANGII AT(1A) and AT(1B) receptors were determined by RNase protection. The low-salt diet increased AT(1A) and AT(1B) receptor mRNA levels by 90% and 220%, respectively. Losartan treatment did not change the basal AT(1A) mRNA level, but decreased AT(1B) mRNA by 50%. Treatment of rats on a low-salt diet with losartan did not change the increase of AT(1A) mRNA but significantly attenuated the increase of AT(1B) mRNA to 90% of the control value. Stimulation of endogenous ANGII levels by unilateral renal artery clipping for 2 days lowered AT(1B) mRNA by 25% and increased AT(1B) mRNA by 30%. Additional treatment with losartan did not affect the decreased AT(1A) mRNA levels in rats with a unilateral renal artery clip, but significantly attenuated the increase of AT(1B) mRNA. These findings suggest that sodium deficiency stimulates adrenal AT(1A) and AT(1B) receptor mRNA levels primarily via an ANGII-AT(1)-independent mechanism. The preferential increase of adrenal AT(1B) mRNA during a low-salt intake could be explained by the elevation of endogenous ANGn levels during sodium deficiency, suggesting that endogenous ANGII acts as an enhancer for adrenal AT(1B) but not for AT(1A) receptor gene expression via ANGII-AT(1) receptors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SUBTYPES; SODIUM; CDNA; CHLORIDE; CELLS; adrenal gland; angiotensin II receptor subtypes; losartan; low-salt intake; renal artery clip |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Armin Kurtz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 23 Feb 2023 10:09 |
| Last Modified: | 23 Feb 2023 10:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49646 |
Actions (login required)
 |
View Item |
