Esser, Ruth and Glienke, Wolfgang and Andreesen, Reinhard and Unger, Ronald E. and Kreutz, Marina and Ruebsamen-Waigmann, Helga and von Briesen, Hagen (1998) Individual cell analysis of the cytokine repertoire in human immunodeficiency virus-1-infected monocytes/macrophages by a combination of immunocytochemistry and in situ hybridization. BLOOD, 91 (12). pp. 4752-4760. ISSN 0006-4971,
Full text not available from this repository. (Request a copy)Abstract
The expression of many cytokines is dysregulated in individuals infected with the human immunodeficiency virus-1 (HIV-1). To determine the effects of HIV-1 infection on cytokine expression in individual cells (at the single cell level), we investigated the intracellular levels of proinflammatory cytokines (tumor necrosis factor [TNF]-alpha, interleukin [IL]-1 beta, IL-6, and IL-8) and hematopoietic growth factors (granulocyte colony-stimulating factor [G-CSF], granulocyte-macrophage colony-stimulating factor [GM-CSF]) in monocyte-derived macrophages, mock-infected, or infected with HIV-1 by immunocytochemical staining for cytokine protein and compared this with secreted cytokine levels as determined by specific enzyme-linked immunosorbent assay (ELISA). No difference in the frequency or intensity of cell-associated immunocytochemical cytokine staining could be observed between HIV-1 and mock-infected cells even though the level of secreted proinflammatory cytokines increased and the hematopoietic growth factors decreased in HIV-l-infected cultures. Furthermore, equal expression of cytokine mRNA was observed in all cells in the culture regardless of whether the cells were productively infected with HIV-1 as determined by double-labelling immunocytochemical staining for HIV-1 p24 antigen and in situ hybridization for cytokine mRNA expression. These results indicate that HIV-1 infection results in dysregulation of intracellular cytokine mRNA expression and cytokine secretion not only in HIV l-infected cells, but also through an indirect way(s) affecting cells not producing virus. (C) 1998 by The American Society of Hematology.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TUMOR-NECROSIS-FACTOR; FACTOR-ALPHA PRODUCTION; ALVEOLAR MACROPHAGES; MONONUCLEAR PHAGOCYTES; MONOCYTES MACROPHAGES; INTERLEUKIN 1-BETA; VIRUS INFECTION; HIV-INFECTION; TAT PROTEIN; INFLAMMATORY RESPONSES; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 May 2023 08:25 |
| Last Modified: | 09 May 2023 08:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/49735 |
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